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| Registros recuperados : 13 | |
| 4. |  | FURINI, G.; REINEHR, J.; COMACHIO, V.; ZILIO, R. A.; MAIA, J. D. G.; SOUZA, R. T. de; RITSCHEL, P. S. Atributos visuais e sensoriais de uvas de mesa cultivadas sob cobertura plástica na Serra Gaúcha. In.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2015. p. 62.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 5. | | REINEHR, J.; FURINI, G.; COMACHIO, V.; ZILIO, R. A.; TAFFAREL, J. C.; MAIA, J. D. G.; RITSCHEL, P. S. Avaliação de atributos físico-químicos e sensoriais de sucos de uva elaborados com nove cultivares de uva. In:CONGRESSO LATINO-AMERICANO DE VITICULTURA E ENOLOGIA, 15., 2015, Bento Gonçalves, RS. p. 379. XV Congresso Latino-Americano de Viticultura e Enologia E XIII Congresso Brasileiro de Viticultura e Enologia. Bento Gonçalves-RS, 3 a 7 de Novembro de 2015.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 6. | | DALAGNOL, L. M. G.; BRUTOLIN, J. G.; COMACHIO, V.; CAMARGO, U. A.; MAIA, J. D. G.; RITSCHEL, P. S. Incidência das principais doenças da videira em acessos mantidos pelo Banco Ativo de Germoplasma de Uva (BAG-Uva). In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 11.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 7., Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2013. p. 55. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 7. | | DALAGNOL, L. M. G.; LORENZINI, M.; COMACHIO, V.; CAMARGO, U. A.; MAIA, J. D. G.; RITSCHEL, P. S. Avaliações agronômicas de acessos mantidos pelo Banco Ativo de Germoplasma de Uva (BAG-Uva). In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 11.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 7., Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2013. p. 54. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | BRUSTOLIN, J. G.; FONTANA, V.; COMACHIO, V.; TONIETTO, J.; FERREIRA, M. E.; MAIA, J. D. G.; RITSCHEL, P. S. Caracterização genética, fenotípica e sensorial da cultivar de videira 'Moscato Branco'. In.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2015. p. 56.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | BRUSTOLIN, J. G.; FONTANA, V.; COMACHIO, V.; TONIETTO, J.; FERREIRA, M. E.; MAIA, J. D. G.; RITSCHEL, P. S. Caracterização genética, fenotípica e sensorial da cultivar de videira ?Moscato Branco?. In.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2015. p. 56.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 10. | | DALAGNOL, L. M. G.; CAUMO, M.; POLONI, T.; COMACHIO, V.; CAMARGO, U. A.; MAIA, J. D. G.; RITSCHEL, P. S. Características do mosto e de compostos relacionados à saúde de acessos mantidos pelo Banco Ativo de Germoplasma de Uva (BAGUva). In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 11.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 7., Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2013. p. 45. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 12. | | BOSCO, D. D.; SINSKI, I.; COMACHIO, V.; MAIA, J. D. G.; FAJARDO, T. V. M.; RITSCHEL, P. S.; QUECINI, V. In vitro establishment and conservation of grapevine germplasm. In: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...] [S.l.]: International Crop Science Society, 2012. Não paginado. 1 pendrive. Resumo 3096.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 13. | | REINEHR, J.; FURINI, G.; LIMA, J. de; ROSSETTO, D.; COMACHIO, V.; ZILIO, R. A.; TAFFAREL, J. C.; MAIA, J. D. G.; RITSCHEL, P. S. Avaliação físico-química e sensorial de sucos elaborados com diferentes cultivares de uva. In.:ENCONTRO DE INICIAÇÃO CIENTÍFICA, 13., 2015. Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2015. p. 63.| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 13 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
05/01/2016 |
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Data da última atualização: |
17/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
BOSCO, D. D.; SINSKI, I.; COMACHIO, V.; MAIA, J. D. G.; RITSCHEL, P. S.; QUECINI, V. |
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Afiliação: |
DANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; VALTAIR COMACHIO, CNPUV; JOAO DIMAS GARCIA MAIA, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; VERA MARIA QUECINI, CNPUV. |
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Título: |
In Vitro techniques for grapevine germplasm conservation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Acta Horticultura, n. 1082, Apr. 2015. p. 201-206. |
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Idioma: |
Português |
|
Conteúdo: |
Abstract Plant genetic resources hold significant phenotypic variation resultant from the presence of allelic diversity, which is maintained by evolutionary processes or by artificial selection. Therefore, plant germplasm encompasses the huge genotypic diversity found in wild and cultivated species and constitutes a source of interesting traits for breeders. Although extremely valuable, biodiversity conservation has high demand for funding, physical space and labor. In vitro conservation is an interesting alternative for the maintenance of highly-heterozygous, vegetatively propagated, perennial species, such as grapevine. It also contributes to the plants? phytosanitary conditions. The current work aimed to develop effective and feasible means toward in vitro establishment and conservation of grapevine germplasm. Woody stakes were obtained from the field collection of the Grapevine Germplasm Bank, at Embrapa, and were surface disinfected, planted in a mixture of autoclaved soil and vermiculite (1:1), and kept under controlled temperature (23±5°C) and relative humidity (70%). Young apical shoots were excised and superficially disinfected in the presence of 1% (w/v) polyvinylpirrolidone. Explants were transferred to tubes containing Galzy medium with active charcoal, under aseptic conditions. Established plants were propagated and maintained in vitro as duplicates. For long-term conservation, the effectiveness of two cryopreservation techniques; vitrification and encapsulationdehydration, was compared for 11 grapevine genotypes, including Vitis vinifera, V. labrusca and hybrids V. berlandieri × V. rupestris, and V. riparia × V. berlandieri. Shoot induction from treated stakes under protected greenhouse conditions significantly reduced environmental contamination and, along with the use of antioxidant agents, allowed in vitro establishment of approximately 1200 (85% of the accessions held by the bank) grapevine accessions. The establishment of the remaining accessions is underway. Plants free of ectophytes were produced for 900 (64.3%) accessions. Cryogenic protocols require further adjustments to allow acceptable recovery rates. High-scale in vitro conservation of grapevine germplasm is feasible and may safeguard valuable biodiversity. Although promising, cryopreservation requires further studies for protocol optimization. MenosAbstract Plant genetic resources hold significant phenotypic variation resultant from the presence of allelic diversity, which is maintained by evolutionary processes or by artificial selection. Therefore, plant germplasm encompasses the huge genotypic diversity found in wild and cultivated species and constitutes a source of interesting traits for breeders. Although extremely valuable, biodiversity conservation has high demand for funding, physical space and labor. In vitro conservation is an interesting alternative for the maintenance of highly-heterozygous, vegetatively propagated, perennial species, such as grapevine. It also contributes to the plants? phytosanitary conditions. The current work aimed to develop effective and feasible means toward in vitro establishment and conservation of grapevine germplasm. Woody stakes were obtained from the field collection of the Grapevine Germplasm Bank, at Embrapa, and were surface disinfected, planted in a mixture of autoclaved soil and vermiculite (1:1), and kept under controlled temperature (23±5°C) and relative humidity (70%). Young apical shoots were excised and superficially disinfected in the presence of 1% (w/v) polyvinylpirrolidone. Explants were transferred to tubes containing Galzy medium with active charcoal, under aseptic conditions. Established plants were propagated and maintained in vitro as duplicates. For long-term conservation, the effectiveness of two cryopreservation techniques; vitrification and encapsulation... Mostrar Tudo |
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Palavras-Chave: |
In vitro; Phytosanitary improvement. |
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Thesaurus NAL: |
Germplasm; Micropropagation; Tissue culture. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136629/1/Ritschel-Actahort-n1082-Apr2015.pdf
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|
Marc: |
LEADER 03023naa a2200241 a 4500
001 2032995
005 2016-03-17
008 2015 bl uuuu u00u1 u #d
100 1 $aBOSCO, D. D.
245 $aIn Vitro techniques for grapevine germplasm conservation.$h[electronic resource]
260 $c2015
520 $aAbstract Plant genetic resources hold significant phenotypic variation resultant from the presence of allelic diversity, which is maintained by evolutionary processes or by artificial selection. Therefore, plant germplasm encompasses the huge genotypic diversity found in wild and cultivated species and constitutes a source of interesting traits for breeders. Although extremely valuable, biodiversity conservation has high demand for funding, physical space and labor. In vitro conservation is an interesting alternative for the maintenance of highly-heterozygous, vegetatively propagated, perennial species, such as grapevine. It also contributes to the plants? phytosanitary conditions. The current work aimed to develop effective and feasible means toward in vitro establishment and conservation of grapevine germplasm. Woody stakes were obtained from the field collection of the Grapevine Germplasm Bank, at Embrapa, and were surface disinfected, planted in a mixture of autoclaved soil and vermiculite (1:1), and kept under controlled temperature (23±5°C) and relative humidity (70%). Young apical shoots were excised and superficially disinfected in the presence of 1% (w/v) polyvinylpirrolidone. Explants were transferred to tubes containing Galzy medium with active charcoal, under aseptic conditions. Established plants were propagated and maintained in vitro as duplicates. For long-term conservation, the effectiveness of two cryopreservation techniques; vitrification and encapsulationdehydration, was compared for 11 grapevine genotypes, including Vitis vinifera, V. labrusca and hybrids V. berlandieri × V. rupestris, and V. riparia × V. berlandieri. Shoot induction from treated stakes under protected greenhouse conditions significantly reduced environmental contamination and, along with the use of antioxidant agents, allowed in vitro establishment of approximately 1200 (85% of the accessions held by the bank) grapevine accessions. The establishment of the remaining accessions is underway. Plants free of ectophytes were produced for 900 (64.3%) accessions. Cryogenic protocols require further adjustments to allow acceptable recovery rates. High-scale in vitro conservation of grapevine germplasm is feasible and may safeguard valuable biodiversity. Although promising, cryopreservation requires further studies for protocol optimization.
650 $aGermplasm
650 $aMicropropagation
650 $aTissue culture
653 $aIn vitro
653 $aPhytosanitary improvement
700 1 $aSINSKI, I.
700 1 $aCOMACHIO, V.
700 1 $aMAIA, J. D. G.
700 1 $aRITSCHEL, P. S.
700 1 $aQUECINI, V.
773 $tActa Horticultura$gn. 1082, Apr. 2015. p. 201-206.
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